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Use a vehicle control: keep the solvent level the same in treated and control samples
Low levels of B12, especially during the first trimester, have been linked to neural tube defects (NTDs) such as spina bifida and anencephaly [*]
Pain reduction becomes significant
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For a 12-week protocol , estimated use increases to approximately 58.8 mg , requiring 6 vials
This is faster than manual calculation and eliminates the transcription errors that happen when converting between milligrams, micrograms, milliliters, and syringe units by hand
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