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For experiments in 384-well plates, 500 cells were seeded per well, and after 24 h, the medium was switched using a series of 4 aspirationwash steps with the desired final medium using Multidrop Combi (Thermo)

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When AOD-9604 binds to or modulates these receptors, it preferentially activates fat cells while having minimal effects on other tissues that primarily express beta-1 and beta-2 adrenergic receptor subtypes

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Our data suggest that tesofensine in rats did not impair sweetness detection or affect its palatability