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We are currently exploring cationic or neutral water solubilizing groups to replace the carboxylic acids in RT to achieve higher temporal resolution while maintaining homogeneous cellular distribution, which will be reported in the due course

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Subsequently, the sections were incubated with secondary antibody (goat anti-rabbit), rinsed with phosphate buffer saline (PBS), stained with DAB, and hematoxylin and eosin (H&E) staining, dehydrated, transparentized, and mounted before microscopic analysis

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