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To confirm transfection efficiency, the unrelated non-essential P

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Diabetologia 59:13721375 Liang Y, Osborne MC, Monia BP et al (2004) Reduction in glucagon receptor expression by an antisense oligonucleotide ameliorates diabetic syndrome in db / db mice

KEY FINDINGS Average weight loss on treatment: 7.9 kg (all GLP-1s) 16.1 kg (newer GLP-1s like semaglutide/tirzepatide) Weight regained after stopping: 0.70.8 kg/month 8.39.6 kg regained in the first year Full return to baseline: 0.9 years (older GLP-1s) 1.7 years (newer GLP-1s) Bottom line: GLP-1s are effective, but without ongoing treatment or structured long-term care, the benefits fade quickly

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They will not fold back into their active configuration