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Closely linked to this, MS techniques, particularly for complex formulations, are highly susceptible to matrix effects that can affect recovery and ionization efficiency of both the peptide and its impurities

Reactive oxygen species (ROS) levels KGN cells were incubated in serum-free DMEM using 3 M C11-BODIPY 581/591 (Invitrogen), washed three times with serum-free medium, trypsinized, resuspended in PBS, and detected by flow cytometry