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Under our conditions, 2.5 M strep-MBP-ROXY9 or 0.2 M strep-MBP-GRXC2 rescued about 6% (0.018 M) of the GAPDH activity that could be recovered with DTT (approximately 0.3 M

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The supernatant was aspirated and the cell pellet was suspended in supplemented DMEM media for further experiments