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Cell culture Bone marrow-derived macrophages (BMDMs) were harvested from the femur and tibia of C57BL/6J mice and cultured in Dulbeccos modified Eagles medium (DMEM) containing 10% fetal bovine serum (FBS, Gibco, Carlsbad, CA, USA) and 10% L929 cell-conditioned medium, and maintained in a humidified incubator with 5% CO 2 at 37 C for 7 days [36]

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AMPK, a key molecule regulating aging-related myocardial ischemia-reperfusion injury

In addition, peptide therapy in general may lead to side effects related to the route of administration, which, in the case of ipamorelin, is subcutaneous injection

Buse, MD, PhD, Distinguished Professor of Medicine, Director of the UNC Diabetes Care Center