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Both cell lines were differentiated into neutrophil-like granulocytes by DMSO treatment, which were checked by CD11b expression (80%) and the presence of dead cells measured by propidium iodide assays (2%), as described [8]

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Interestingly, after Pycnogenol intake, none of the hormone levels showed significant changes compared to baseline or placebo, suggesting non-hormonal mechanisms of Pycnogenol on climacteric symptoms

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