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FIGURE 8 3.8 GHK-Cu inhibited the expression of RORt in DSS-induced colitis Western blot analysis showed that treatment with GHK-Cu significantly reduced DSS-induced RORt expression (Figure 9), implying Th17 cell inhibition contributes to its therapeutic effect

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Cys 2 ), cystine-free (Cys 2 -free), or cystine-free/GSH-supplemented (750 M) medium for the indicated timepoints, and cell numbers were determined (n = 2 technical replicates representative of one experiment)