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DOI: Article Snippet: Purified PBMCs, at a density of 1.5 10 6 cells/mL, were seeded in a flask (Corning, 430,168) coated with scFvCD16A-sc4-1BBL (2 g/ml, 24 h at 4 C), and cultured in KBM-581 medium (Corning, 8851-CM) containing 5% heat-inactivated autologous plasma and 1000 IU/mL rhIL-2 (Jinsili, Jiangsu, China) for 13 days at 5% CO 2 and 37, by adding fresh medium every 23 days to maintain the cell density between 1.3 and 3.2 10 6 cells/mL. Cao, G., Zhang, G., Liu, M., Liu, J., Wang, Q., Zhu, L., & Wan, X

At the end of this 7-day labelling period ( t = 0 h), samples were collected to verify complete incorporation of the heavy label into cellular proteins

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