The filter was washed two times with 100 L of UA, centrifuged at 14,000 g for 15 min, and incubated with 100 L of IAA solution (0.05 M iodoacetamide in UA) for 20 min in the dark
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The selenenic acid is then converted back to the selenol (RseH) by a two-step process that begins with a reaction with GSH to form the GS-SeR and water
This alteration results in the loss of GST expression, and is proposed to occur during pathogenesis of the disease (Lee et al ., 1994)
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