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Apoptosis was evaluated after 4 and 24 h of treatment using the Promega apoptosis assay kit (JA1011), according to the manufacturers protocol

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cDNA clones of AsGGT1 , AsGGT2 , and AsGGT3 were re-isolated by RT-PCR using KOD plus DNA polymerase (Toyobo, Osaka, Japan) and the following primers: AsGGT1-F (5-TCATATTCTGACGCAGATTCCACAG-3) and AsGGT1-R (5-TGTTCAATCATATTTTGTACAAATAGAC-3) for AsGGT1