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Nonaromatic amino acids with either nitrogen-containing or oxygen-containing side chains and ,-disubstituted amino acids were not well tolerated by Cp1B and Cp2B (Supplementary Fig

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The measurement of paracellular permeability is usually quite straightforward since the movement of ions and charged organic molecules can be measured as an electrical current and the movement of neutral molecules can be measured by assaying their concentration in the bloodstream or urine or in a compartment equivalent to the subepithelial space in mounted or perfused tissue or in cultured cells grown on permeable substrates