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HUVEC cells were stained with 0.5 M Calcein-AM and the fluorescence was detected by fluorescence microscope, and quantitative analysis of each image was performed using Image J 1.53t software

MT, SA, and DS analyzed the diet data

inject slowly and steadily

Advanced Protocol Considerations Some researchers explore higher doses in the 2 to 5 mg daily range under medical supervision for more intensive skin remodeling needs

Injection site reactions usually fade within 30 to 60 minutes

The cells were then centrifuged at 10,000 g for 2 min and incubated with 1 ml of 2 M HCl and 0.5% Triton X-100 (Sigma-Aldrich, 93443) for 30 min with gentle agitation