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To quantify total PDHc-E2 and OGDHc-E2, lipoate blots were stripped using Restore Western Blot Stripping Buffer (Thermo Fisher Scientific, 21059), reblocked in 5% skim milk in PBST and re-immunoblotted using appropriate primary antibodies

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In this state, the particles do not migrate in an electric field, a condition known as the isoelectric state

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