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After washing three times with TBS, slides were processed using the Vector ABC kit (Vector Laboratories, Inc., Burlingame, CA, USA)
Darrow JJ, Kesselheim AS

Anderson-metastatic breast-231) MG-63 human osteosarcoma cell line Mia PaCa-2 human pancreatic carcinoma cell line MIC minimum inhibitory concentration M micromolar MMP-9 matrix metalloproteinase-9 MRSA methicillin-resistant Staphylococcus aureus MS mass spectrometry MTS 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide NAC N-acetylcysteine NF-kB nuclear factor kappa-light-chain-enhancer of activated B cells nm nanometer NMR nuclear magnetic resonance NRF2 nuclear factor erythroid 2-related factor 2 QTOF quadrupole time-of-flight P27 cyclin-dependent kinase inhibitor 1B P53 tumor protein p53 gene PARP poli(ADP-ribosa) polimerasa PC-3 human prostate cancer cell line PCA principal component analysis PDT photodynamic therapy pH hydrogen potential PNT-2 human prostate cell line PTA 1,3,5-triaza-7-phosphaadamantane RAPTA ruthenium arene PTA RAPTA C [RuII(6-p-cymene)Cl2(pta)] ROS reactive oxygen species RPMI 1640 Roswell Park Memorial Institute 1640 medium SCXRD single-crystal X-ray diffraction SI selectivity index SK-OV-3 human ovarian cancer cell line SLC5A6 solute carrier family 5 member 6 (gene) SRB sulforhodamine B SW-620 human colorectal adenocarcinoma cell line T-47D human breast cancer cell line T-89G human glioblastoma cell line UHPLC ultra-high-performance liquid chromatography U-87 Uppsala 87 malignant glioma UVVis ultraviolet-visible spectroscopy Vero African green monkey kidney cell line VRSA vancomycin-resistant Staphylococcus aureus WHO World Health Organisation

While these renal markers returned to baseline after a recovery period (washout), the data suggests that Adipotide research requires careful monitoring of renal function markers (BUN and creatinine) and hydration status
Senescence, a cellular response to stress and other damage (Franceschi and Campisi, 2014)