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Online 39 , 332342 (2019)

Sequencing was performed using a HiSeq 2500 (Illumina), with a read length of 300 bp paired-end reads and at least 25,000 tags per sample

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Of significance and supporting a potential anti-cancer action of metformin is that LKB1 is a tumor suppressor and that mutations in LKB1 are observed in numerous cancers that result in the reduction of the inhibitory effect of the LKB1/AMPK pathway on the pro-proliferative signaling via mTOR (125, 126)

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