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[Cellular localization, half-life, and secretion of peptide YY]

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The supernatant from the hypotonic solution was combined with the supernatant from the hypertonic solution, centrifuged to remove debris, and dialyzed against 20LmM Tris, pH 7.5 and 150LmM NaCl (Tris-buffered saline, TBS) overnight at 4 C

Deletion analysis has revealed that the essential portion of the promoter is only about 150 base pairs long