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The protein was purified through heparin affinity chromatography using 50 mM sodium citrate buffer, pH 6.0, then processed using ion-exchange chromatography (IEC), and finally purified by SEC on a Superdex 75 column (HiLoad 16/600, Cytiva)
121 However, studies on endothelial function after inhibition of xanthine oxidase have provided equivocal results in hypertension models
A few trials, though, just showed sleep improvement with no effect on behavior.[ref][ref] A study with 125 autistic children with sleep problems found that melatonin use increased sleep onset time (bedtime) by almost an hour per night and decreased the amount of time that it took to fall asleep
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