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The serum samples were kept at -20C pending biochemical determinations which included total testosterone, free testosterone, 8-hydroxy-2-deoxyguanosine (8-OHdG) and advanced glycation end products (AGEs) that were measured by enzyme linked immunosorbent (ELIZA) using ELIZA kits (Sunlong, China, Catalogue Number SL1061Ra, SL0293Ra, SL0019Ra, SL0036Ra respectively) according to the operatings instructions

administered quercetin, luteolin, and octyl gallate to C57BL/6 J mice for three days before inoculation with C

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Homemade solutions without preservatives: Must refrigerate immediately Use within 1-2 weeks maximum Risk of bacterial contamination is high Consider adding preservative system Homemade solutions with preservatives: Refrigeration recommended Can last 1-3 months refrigerated Room temp storage reduces to 2-4 weeks Raw GHK-Cu powder mixed into existing products: Follow storage requirements of base product Generally refrigerate to be safe Use within original product's timeframe Learn more about copper peptide formulations in our copper peptides complete guide

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Throughout this period, the favorable safety profile remained consistent with no delayed-onset adverse effects or concerning laboratory changes emerging over time