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Reference: Sikiric P, Seiwerth S, Grabarevic Z, et al

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Preparation of protein lysates The cells collected from 6-well plates were lysed in 0.4 ml lysis buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, and 2% SDS) followed by sonication (10 cycles of 10 s pulses with 10 s rest intervals) using a Fisher Scientific Sonic Dismembrator Model 500 at 15% amplitude