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MS1 parameters were as follows: spray voltage: 3500 V for positive ionization and 2500 V for negative ionization modes, sheath gas: 40, auxiliary gas: 10, sweep gas: 1, ion transfer tube temperature: 300C, vaporizer temperature: 350C, orbitrap resolution: 120K, scan range(m/z): 250-2000 for pos, 200-2000 for neg, RF lens(%): 60, automatic gain control (AGC) target: 50%, and a maxIT of 100 milliseconds (ms)

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doi: 10.3390/metabo12111059 18

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In some cases, its presence can affect the stability or bioactivity of the peptide