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400 l of the reagent mixture (30 ml glacial acetic acid, 20 ml phosphoric acid and 1.25 g ninhydrin) was mixed with 200 l supernatant and heated in sealed test tubes at 100 C for 1 h

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And will participants have access to the treatment after the study

10.1093/bja/aev226 21

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Results Search Results and Study Characteristics The search identified a broad and heterogeneous body of literature spanning multiple peptide agents and a wide range of non-orthopaedic indications