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Blot-based detection of dehydroalanine-containing glutathione peroxidase with the use of biotin-conjugated cysteamine

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Under normal conditions, this O 2 is cleared to hydrogen peroxide (H 2 O 2 ) by superoxide dismutase (SOD) in the mitochondrial matrix [23]

The disulfide bonds in certain proteins are oxidized by ROS which can affect their activity, especially in the tyrosine phosphatases superfamily [25]

Diabetologia 52 , 199207 (2009)