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On day 35, the rats were fasted for 6 h starting at 08:00 h and underwent a one-step hyperinsulinaemic-euglycemic clamp using a constant infusion of 3 H-glucose as a tracer (60-min equilibrium, 30-min baseline, 120-min clamp) to determine glucose uptake and hepatic glucose production (HGP)
2024;32(S1):55308
Mouse islets were transduced 24 hours prior to imaging with an adenoviral SNAP/FLAG-hGLP-1R vector (custom made by VectorBuilder) at a MOI of 1 and subsequently labelled for 10 minutes at 37 C with SNAP-Surface Alexa Fluor 647 before 5-minute stimulation with indicated agonists and imaging of whole islets in the same microscope as above
Aim to incorporate: Dark, leafy greens (e.g., broccoli, kale, Brussels sprouts, Swiss chard), Red and orange vegetables (e.g., bell peppers, winter squash, sweet potatoes) Beans, peas, and lentils (e.g., pinto beans, cannellini beans, red lentils, dal)
Biochemistry and molecular cell biology of diabetic complications
J.RosenstockJ.Perez ManghiF
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