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The effects of retatrutide on FOC were also reduced by 100 nM ryanodine, an inhibitor of the ryanodine receptor in the sarcoplasmic reticulum, by 1 M carbachol, a M-cholinoceptor agonist, and by 1 M (-)-N 6 -phenylisopropyladenosine, an A 1 -adenosine receptor agonist

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This involved perfusing the brains with phosphate-buffered saline followed by 4% paraformaldehyde, sectioning them into eight-micrometer coronal slices, and visually confirming the cannulas location within the left lateral ventricle [1]