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Cell Viability Analysis Cell viability was evaluated using a Premix WST-1 Cell Proliferation Assay System (TaKaRa Bio Incorporated) according to the manufacturers instructions

Common side effects for both: Nausea (most common, improves over time) Diarrhea or constipation Vomiting (less common) Abdominal pain or bloating Reduced appetite (this is intended, but can be extreme) Fatigue, especially in first month Managing GI side effects: Eat smaller, more frequent meals Avoid fatty, greasy, or spicy foods Stay hydrated Don't lie down immediately after eating Consider anti-nausea medication if severe Most side effects peak in weeks 2-4 of each new dose, then improve significantly

This years theme, Food Access and Healthy Living, reflects a sharpened focus on two of the most pressing needs identified in the Wellstar 20252028 Community Health Needs Assessment and Implementation Plans

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Epidemiology of Insomnia: Prevalence, Course, Risk Factors, and Public Health Burden