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Injections are typically administered once weekly on a consistent day, with dosage increasing gradually over time based on tolerance and response

A six-week gap means starting over with titration

A gradual decline in energy production out of the mitochondria is a hallmark of aging

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Real-time polymerase chain reaction (PCR) was performed by using a kit (SYBR Premix EX Taq, Takara Bio Inc.) and the ABI PRISM 7300 real-time PCR system

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