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For this, pelleted cells were thawed and resuspended in binding buffer (20 mM Tris-HCl pH 7.0, 300 mM NaCl, 2 mM -mercaptoethanol) supplemented with 0.2 mM serine protease inhibitor AEBSF-HCl (Carl Roth GmbH, cat no
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The UFS-QOL, a new disease-specific symptom and health-related quality of life questionnaire for leiomyomata