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Transwell assay The chemotactic motility of HUVECs was determined using transwell migration chambers (Corning) with 6.5 mm diameter polycarbonate filters (8 m pore size), as described previously
I also - wimp that I am - take a pain killer beforehand which helps though whether that is all in my mind or not I don't know
TB500 is investigated for its association with actin polymerization dynamics and intracellular actin sequestration, which directly influences cell motility, differentiation, and structural reorganization