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FIGURE 2 In addition to be influenced by total intake of TMA precursors, TMAO levels can also be reduced by some bioactive compounds in nutrients

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The receptor constructs were fused with a human IgG leader (MGWSCIILFLVATATGVHSE) at their N-termini for cell membrane localization, and a 3xFLAG tag (DYKDDDDKDYKDDD-DKDYKDDDDKV) at their C-termini for immunoblotting

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Urinary NO x (sum of nitrate and nitrite) excretion in response to intraperitoneally administered lipopolysaccharide (LPS) was identical in the control and cystine group (data not shown), demonstrating equal host capacity to produce NO