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Serum limitation-induced senescent fibroblasts model and effect of FOXO4-DRI Passage 3 fibroblasts derived from keloids and normal skins were cultured in a Dulbeccos Modified Eagles medium (DMEM, Hyclone, Logan, Utah, USA) containing 10% fetal bovine serum (FBS, Gibco, Grand Island, NY, USA) to 90% confluence, and then cultured in a DMEM containing 2% FBS for 3 days to construct a serum limitation-induced senescent fibroblast model

Proc Natl Acad Sci USA 2012;109:53227

Bioavailability is lower than subcutaneous and the human evidence is thinner

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GHK-Cu fits into that picture naturally because it addresses the same biological process from a different angle, restoring the signal instead of forcing the response through physical injury

Its then medical director Professor Stephen Powis called wellness drips reckless and exploitative and said healthy people simply do not need them