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Electron spin resonance spectroscopy For the analysis of in vitro samples, reactants, including the spin trap, were mixed in PBS containing diethylenetriaminepentaacetic acid (DTPA, 50 M) to a final volume of 50 l, using low protein binding tubes (90410, Thermo Fisher Scientific)

Thermodynamic studies reveal that MT3 binds Cu + through enthalpy-driven processes and Zn + via entropy-driven mechanisms, forming Cu 4 thiolate clusters

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Allow the alcohol to dry completely before injecting, as injecting through wet alcohol can cause stinging