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& Boissy, R
Ce-DAB2 staining and preparation of Drosophila larval CNS for EM The dissected flies central brain and VNC were rinsed (five times for 3 min each) with 0.15 M sodium cacodylate buffer pH 7.4 containing 2 mM CaCl 2 on ice and blocked with 20 mM glycine, 50 mM potassium cyanide and 50 mM aminotriazole in 0.15 M sodium cacodylate buffer pH 7.4 containing 2 mM CaCl 2 for 20 min on ice
Discussion Mounting evidence implicates that obesity represents a state of long-term, low-grade inflammation, and ATMs are a significant contributor to systematic inflammation and dysfunctional insulin action in obese individuals [51]
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