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Wait 5 to 10 seconds before withdrawing the needle to allow the liquid to disperse

Preparation of working reagents KH 2 PO 4 (0.2 M) and Na 2 PO 4 (0.2 M) were mixed at a ratio of 40.5:9.5, (v/v), and the pH, if necessary, was adjusted to 7.4 by adding KH 2 PO 4 or Na 2 HPO 4 solutions drop-wise

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