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Images were taken at 100 magnification and the red staining (representing the proteoglycans) in the core portion of the ligament was quantified with BIOQUANT OSTEO software, expressed as percentage of the proteoglycan (red-stained) area over the total area

2.1 Genomic findings Genomic and bioinformatic analyses followed a standardized workflow including QiAamp DNA extraction, Illumina DNA Prep with Exome 2.5 Enrichment, and XLEAP-SBS sequencing at ~100 coverage

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