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Secondary antibodies were Alexa Fluor 488 goat anti-mouse IgG or Alexa Fluor 568 goat anti-rabbit IgG from Life Technologies (both 1:400)

The definitive K i values reported in this study are obtained from nonlinear regression analysis, where kinetic parameters are calculated using all substrate and inhibitor concentration data simultaneously

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Try cycling supplements (4 days on, 3 days off), taking them every other day, or fasting for 16-24 hours while working with your healthcare practitioner