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The original Western blots are included as Source Data File

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In short, a commercially available calibration kit for low and high molecular weight (MW) proteins (Cytiva Germany GmbH, Freiburg DE) was used to calculate elution volumes ( V e ) of respective standard proteins

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Tested compounds were pre-incubated for 1 h with 45 nM of human PCSK9 before being transferred to the cell plates