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Sources Primary Research Interviews Healthcare Practitioners (Endocrinologists, Diabetologists) Clinical Trial Coordinators Pharmaceutical Executives Patient Advocacy Groups Regulatory bodies (e.g., U.S

coli XL1-blue using heat shock transformation followed by mini prep using peqGOLD plasmid MiniPrep kit (VWR International) for the confirmation of the transformation using restriction enzyme digestion ( Sma I and Hin dIII) of the plasmid followed by agarose gel electrophoresis

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Protein : Adequate protein intake supports both muscle and bone health during weight loss