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ATPase activity assays For evaluating ATPase activity, WT and E1435Q Ycf1 were expressed and purified as described above in buffer containing 0.01% LMNG and 0.001% CHS

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For each individual AA the protein-bound form decreases to a highly similar extent during lactation, indicating that the dynamics of protein-bound AAs in human milk during lactation are not AA-specific (55, 56)

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