two adjacent aspartic acid residues at positions 10 and 11 create a localized acidic region at physiological pH
This doesnt mean B12 causes glaucoma, but it flags a potential risk of high-dose supplements in those predisposed
(PubMed) Mares-Perlman JA, Brady WE, Klein BE, et al
It is also important to minimize the potential presence of proteases in protein purification, either from sources intrinsic to the production process (e.g., host-cell proteins) or from extrinsic sources of contamination (e.g., adventitious microbes)
Now, moving on with the review
Supplementary Movie (download MP4 Non-invasive in vivo fluorescence imaging of ICG 4 -GS-Au25 in PBS-treated and DEM-treated mice