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The resulting pellet was re-suspended in minimal 50 mM HEPES pH 8.0, 200 mM NaCl, applied to a 1545% w/v sucrose gradient and subjected to ultracentrifugation at 4 C for 3 h, 105,000 g

Gels 9 (5), 362

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1c) and preliminary mutational studies on Tyr110 show indeed similar effects to Lys105 (Supplementary Figs 18 and 19)

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For gene ontology (GO) analysis, the Seurats FindMarkers output for the specified groups was used to plot gene ontology terms with the barcodeplot functions from limma 131