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Starr JI, Mako ME, Juhn D, Rubenstein AH (1978) Measurement of serum proinsulin-like material: cross-reactivity of porcine and human proinsulin in the insulin radioimmunoassay

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Mice were decapitated, their brains were collected and immediately submerged in ice-cold, carbogen-saturated (95% O 2 and 5% CO 2 ) artificial cerebrospinal fluid (aCSF