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Affinity purified protein samples were further resolved by size exclusion chromatography and assessed by SDS-PAGE and negative stain TEM before vitrification and cryo-EM imaging (Supplementary Fig

the areal density of the stained mucosa was examined by TEM

The resulting MS raw files were processed with the DIA-NN software v.1.876 in a library-free manner, using a Mus Musculus FASTA file from UniProt (December 2023)

- Use appropriate reaction conditions and reducing agents (such as triethylamine-trimercaptopropane) to promote the formation of disulfide bonds between cysteine and other amino acids

They educe nausea or digestive discomfort and support steady, healthy weight loss

Septic shock is a life-threatening condition characterized by profound circulatory and cellular/metabolic abnormalities that substantially increase mortality, with rates approaching 40%-60% in some cases