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Retrieved from Liu, X., Yang, T., & Liu, H

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(24) demonstrated that HSV entry into Vero cells occurs vial fusion at the plasma membrane and is inhibited by acid pretreatment of virions (pHs 4.7 to 6.0 in HEPES buffer), therefore it is possible that adequate concentrations of vaginal lactic acid are able to block the early stages of HSV-2 infection

Trehalose and mannitol serve as effective cryoprotectants, while phosphate buffers maintain pH stability throughout processing

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