2.3 Performing gel electrophoresis According to the results of BCA, the sample volume of the target protein was 20-40g per well, and the sample volume of the internal reference protein was 5-10g per well
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The levels of kidney function markers, kidney injury molecules-1 (KIM-1), metallothionein, lipid peroxidation, nitric oxide, tumor necrosis factor-, interleukin-1, and the apoptosis regulators Bax and caspases-3 also increased significantly in the renal tissue of Cd-treated mice, whereas the levels of glutathione, antioxidant enzyme activities, and the apoptosis inhibitor Bcl-2 were significantly reduced in the renal tissue of Cd-treated group
Mathalon DH, Rapoport JL, Davis KL, Krystal JH (2003)