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It also plays a role in the hair follicle regression and recovery cycle, and deficiency has been directly linked to both telogen effluvium and diffuse alopecia

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The thermal cycling comprised an initial denaturation step at 95 C for 4 min, followed by 35 cycles at 95 C for 30 s, 59 C for 30 s and 72 C for 30 s, with the final extension at 72 C for 7 min

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