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Animal model research reveals remarkable efficacy in both accelerated and natural aging conditions, with XpdTTD/TTD mice (accelerated aging model) showing restoration of multiple age-related deficits including fitness, fur density, and renal function following FOXO4-DRI administration

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Cell viability relative to untreated controls was quantified using a standard Cell Counting Kit-8 (CCK-8) assay (YEASEN, Shanghai, China)

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