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whereas given a low K m value for L-arginine as substrate of NOS (1020 M), arginase may not affect basal NO production, unless L-arginine level falls below 1020 M (Buga et al., 1996)

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Prior to any procedure, animals are anesthetized using a combination of xylazine (10 mg/kg), ketamine (40 mg/kg) and acepromazine (0.75 mg/kg) for up to an hour via the intramuscular route

Always follow up with a good moisturiser

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Samples from different conditions are combined early in processing, with relative protein abundance determined by heavy-to-light peptide signal ratios (Peters-Hall et al., 2015)