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The enriched IGF-1/LR3 eluate is dried in a speed vac to remove TFA, and then reconstituted for subsequent reduction, alkylation, and tryptic digestion

After performing automatic shimming using the Fast Automatic Shim Technique with Echo-planar Signal readout for Mapping Along Projections (FASTEST MAP, Siemens prototype sequence), manual shimming was performed to make the linewidth of the water spectrum smaller than 20 Hz

J Neuro Neurophysiol 2023, 14 (3), 001-008 Received: 23-Mar-2023, Manuscript No

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